GC-MS profiling of Volatile Metabolites produced by Enterococcus faecalis and Evaluation of Its Antibacterial and Antifungal Activity
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Enterococcus faecalis, Secondary metabolites, Antibacterial, GC/MS
Abstract
Aims and Objectives:
The purpose of this laboratory study was to study the truly biologically active chemical compounds produced by Enterococcus faecalis and to evaluate the antibacterial and antifungal bioactivity of these products.
Method:
In this research, (GC-MS) techniques were used to investigate the biochemical components that are commonly referred to as bioactive substances. At the same time, these bioactive substances are referred to as bioactive secondary metabolites. Furthermore, the ethanolic extract of Enterococcus faecalis was tested in an experimental laboratory to determine whether it actually has effective antibacterial and antifungal properties.
Results:
Using GC-MS analysis on Enterococcus faecalis, the presence of the following bioactive components was experimentaly identified: 2-methyl-1-propenol, 2-pentene, 4-methyl-2,4-diphenyl, 2,4-methyl Dioxo-4-phenylbutanoate, 2 -pentnone, -phenylbutan-2-yl acetate, methyl 2-(butn-2-ylsulfanyl)acetate, trithylene glycol bis(2-ethyl butyrate), methyl 2-(butan-2-ylsulfanyl)) Acetate, triethylene glycol PS (2-ethyl butyrate), 3-mercapto-2-pentenone, ethyl 2-ethylhexanoate, 3-methylbutyl acetate. Metabolites of Enterococcus faecalis showed significant activity against Proteus mirabilis (20.18 ± 0.04). Metabolites of Enterococcus faecalis were highly active against Fusarium oxyporum (13.76 ± 0.17).
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References
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